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Autoimmunity -HLA B27 PDF Print E-mail
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Saturday, 23 August 2008 09:23

Reagent for HLA-B27 Antigen Determination

Human Leukocyte Antigen B27, a class I surface antigen encoded by the B locus in the major histocompatibility complex (MHC) presents microbial antigens to T-Lymphocytes. The onset of seronegative spondyloarthropathies that includes ankylosing spondylitis, Reiter's disease, psoratic arthritis and inflammmatory bowel disease is associated with the expression of HLA-B27 antigen on T-Lymphocytes.

The ReaPan B27 Reagent from ReaMetrix is a dried-down two-color immunofluorescence stain, provided as a ready-to-use test. It is suitable for determining HLA-B27 antigen expression by flow cytometry in lysed human whole blood samples (lyse-no wash technique). This reagent is intended for specific use on open system flow cytometers (e.g. BD FACScan and BD FACSCalibur cyometers).

Key Features of ReaPan B27 Reagent

  • Convenience- Provided as a dried-down reagent in a ready to use format, requiring minimal pipetting.

  • Stability - Dried-down reagent does not require cold storage. Fixed and stained samples can be stored for up to 7 days prior to analysis.

  • Simple Sample Workflow - Reagents employ simple protocol of lyse/no wash protocol.

  • Improved Specificity - The presence of B7 antibody in the reagent reduces false positives due to cross-reactivity of B27 antibody with B7 antigen.

Example of Assay Workflow

ReaPan HLA B27 Sample Data

Product List



Part Number
Package Size





ReaPan B27
50 Tests


HLA ABC.m3, BB7.1



Last Updated ( Wednesday, 26 November 2008 22:11 )
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